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Published on: September 24, 2015
Quantum dot-based western blot technology for ultrasensitive detection of tracer proteins
Rumiana Bakalova1, Zhivko Zhelev, Hideki Ohba
1On-site Sensing and Diagnosis Research Laboratory, AIST-Kyushu, 807-1 Shuku-machi, Tosu 841-0052, Japan. r.bakalova-zheleva@aist.go.jp
Journal of the American Chemical Society
|June 30, 2005
Abstract:
The present study describes two quantum dot-based Western blotting protocols for ultrasensitive detection of "tracer" proteins in cell lysates.
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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
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