Related Experiment Video
Updated: Jul 17, 2026

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
Published on: December 20, 2017
Cell-penetrating cis-gamma-amino-l-proline-derived peptides
Josep Farrera-Sinfreu1, Ernest Giralt, Susanna Castel
1Barcelona Biomedical Research Institute, Combinatorial Chemistry Unit, Barcelona Science Park, University of Barcelona, Spain.
Abstract:
The synthesis of cis-gamma-amino-l-proline oligomers functionalized at the proline alpha-amine with several groups that mimic the side chains of natural amino acids, including alanine, leucine, and phenylalanine, is herein described. These gamma-peptides enter into different cell lines (COS-1 and HeLa) via an endocytic mechanism. The ability of these compounds to be taken up into cells was studied at 37 degrees C and 4 degrees C by plate fluorimetry, flow cytometry, and confocal microscopy. In addition to their capacity for cellular uptake, these unnatural short length oligomers offer advantages over the well-known penetrating TAT peptide, such as being less toxic than TAT and protease resistance.
More Related Videos
Related Concept Videos
Protein Glycosylation
Glycosylation occurs in...
Protein Folding Quality Check in the RER

