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Rattus norvegicus melanocortin 3 receptor: a corrected sequence

Derek Daniels1, Aae Suzuki, Edan Shapiro

  • 1Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce Street, 220E, Philadelphia, PA 19104, USA. derekd@vet.upenn.edu

Peptides
|June 30, 2005
PubMed

Insights

Researchers clarified the rat melanocortin 3 receptor (MC3R) sequence using RT-PCR, finding it differs from the published version by three amino acids in the transmembrane domain. This study highlights the value of genomic data for receptor research.

Area of Science:

  • Genomics
  • Molecular Biology
  • Pharmacology

Background:

  • The rat melanocortin 3 receptor (MC3R) sequence previously published (accession X70667) showed discrepancies with the Rattus norvegicus genome.
  • Clarification of the MC3R sequence is crucial for understanding its function and interactions.

Purpose of the Study:

  • To resolve discrepancies in the rat MC3R sequence.
  • To characterize the functional properties of the clarified rat MC3R.

Main Methods:

  • RT-PCR was used to clone the MC3R from Sprague Dawley rats.
  • Sequence analysis was performed to compare the cloned MC3R with the published sequence and genomic predictions.
  • Transient expression studies were conducted to assess receptor binding and signaling.

Main Results:

  • The cloned rat MC3R sequence aligns with the rat genome but differs from the published sequence by three amino acids in the second transmembrane domain (TM2).
  • Analysis revealed increased homology of rat MC3R TM2 with other species.
  • The clarified MC3R sequence maps to rat chromosome 3, showing conserved synteny with mouse chromosome 2 and human chromosome 20.
  • Functional assays demonstrated high-affinity binding and a concentration-dependent cAMP response to a synthetic agonist.

Conclusions:

  • The study provides a clarified and accurate sequence for the rat MC3R.
  • The findings underscore the utility of genomic information in refining molecular and functional characterization of receptors.
  • The clarified MC3R sequence and its functional data are valuable for future research in melanocortin signaling.

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