Related Experiment Videos
Rattus norvegicus melanocortin 3 receptor: a corrected sequence
Derek Daniels1, Aae Suzuki, Edan Shapiro
1Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce Street, 220E, Philadelphia, PA 19104, USA. derekd@vet.upenn.edu
Peptides
|June 30, 2005
Summary
Researchers clarified the rat melanocortin 3 receptor (MC3R) sequence using RT-PCR, finding it differs from the published version by three amino acids in the transmembrane domain. This study highlights the value of genomic data for receptor research.
Area of Science:
- Genomics
- Molecular Biology
- Pharmacology
Background:
- The rat melanocortin 3 receptor (MC3R) sequence previously published (accession X70667) showed discrepancies with the Rattus norvegicus genome.
- Clarification of the MC3R sequence is crucial for understanding its function and interactions.
Purpose of the Study:
- To resolve discrepancies in the rat MC3R sequence.
- To characterize the functional properties of the clarified rat MC3R.
Main Methods:
- RT-PCR was used to clone the MC3R from Sprague Dawley rats.
- Sequence analysis was performed to compare the cloned MC3R with the published sequence and genomic predictions.
- Transient expression studies were conducted to assess receptor binding and signaling.
Main Results:
- The cloned rat MC3R sequence aligns with the rat genome but differs from the published sequence by three amino acids in the second transmembrane domain (TM2).
- Analysis revealed increased homology of rat MC3R TM2 with other species.
- The clarified MC3R sequence maps to rat chromosome 3, showing conserved synteny with mouse chromosome 2 and human chromosome 20.
- Functional assays demonstrated high-affinity binding and a concentration-dependent cAMP response to a synthetic agonist.
Conclusions:
- The study provides a clarified and accurate sequence for the rat MC3R.
- The findings underscore the utility of genomic information in refining molecular and functional characterization of receptors.
- The clarified MC3R sequence and its functional data are valuable for future research in melanocortin signaling.