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In Ovo Intravascular Injection in Chicken Embryos
Published on: June 3, 2022
[Generation of Chicken Germ-line Chimeras by Transferring PGCs and Their Identification by AFLP.]
Xiao-Fen Hu1, Bei Xie, Rui-Song Yu
1Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai 201106, China,
Yi Chuan = Hereditas
|June 30, 2005
Abstract:
PGCs (Primordial germ cells) were isolated from the blood of 51~56 h hatching Shiqiza chicken embryos by Ficoll density gradient centrifugation. The PGCs were injected into 2.5 d hatching embryos of H breed chicken to produce germ-line chimeras. AFLP checking method was established to identify chicken germline chimeras. Eight germ-line H-S chimera embryos were identified among 20 developing H breed embryos.
