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Related Experiment Videos

Traceability in fluorometry: Part II. Spectral fluorescence standards.

U Resch-Genger1, D Pfeifer, C Monte

  • 1Federal Institute for Materials Research and Testing, Working Group Optical Spectroscopy, Division I.3, Berlin, Germany. ute.resch@bam.de

Journal of Fluorescence
|June 30, 2005
PubMed
Summary

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Accurate fluorescence measurements require traceable spectral standards for instrument calibration. This study presents chemical standards for characterizing fluorescence instruments, ensuring reliable spectral data across various systems.

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Metrology

Background:

  • Accurate characterization of fluorescence instruments is crucial for reliable chemical analysis.
  • Existing methods for spectral correction often lack traceability and can be complex.
  • Sources of error in fluorescence measurements necessitate robust calibration procedures.

Purpose of the Study:

  • To emphasize the need for traceable characterization of fluorescence instruments from a chemist's perspective.
  • To present spectral fluorescence standards for determining wavelength- and polarization-dependent responsivity and irradiance.
  • To demonstrate simple procedures for obtaining corrected emission spectra with acceptable uncertainties.

Main Methods:

  • Identification of major error sources in fluorescence measurements and instrument calibration.

Related Experiment Videos

  • Evaluation of physical and chemical transfer standards for spectral correction curve generation.
  • Development and application of traceable chemical emission and excitation standards.

    • Main Results:

    • Established procedures for the traceable characterization of fluorescence instruments using chemical standards.
    • Demonstrated the utility of dye-based standards across a broad spectral region.
    • Presented methods applicable to spectrofluorometers, other fluorescence systems, and Raman spectrometers.

    Conclusions:

    • Traceable chemical spectral standards are essential for accurate fluorescence instrument characterization.
    • The presented procedures offer a reliable method for generating corrected emission spectra.
    • These methods enhance the comparability and accuracy of fluorescence and Raman spectroscopy data.