Performance validation for microplate fluorimeters
Related Experiment Video
Updated: Aug 17, 2026

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
Published on: December 12, 2011
Robert Giebeler1, Evelyn McGown, Todd French
1Molecular Devices Corporation, 1311 Orleans Drive, Sunnyvale, CA 94089, USA. Bob_Giebeler@moldev.com
Abstract:
As the sophistication of instruments that make fluorimetric measurements on samples in microplates has increased, so has the need for methods to validate instrumental performance. This paper describes a solid-state validation microplate that tests multiple aspects of fluorescence performance, including signal linearity, gain, noise, sensitivity, wavelength accuracy, and polarization stability. Both the operating principles and the validation of the validation microplate are discussed.

