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Related Experiment Videos

Alive and dead Lactobacillus rhamnosus GG decrease tumor necrosis factor-alpha-induced interleukin-8 production in

Liyan Zhang1, Nan Li, Ricardo Caicedo

  • 1Department of Pediatrics, College of Medicine, University of Florida, Gainesville, FL 32610-0296, USA.

The Journal of Nutrition
|July 1, 2005
PubMed
Summary
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Heat-killed Lactobacillus rhamnosus GG (LGG) probiotics effectively reduce inflammation by downregulating interleukin-8 (IL-8) production. Live LGG probiotics also reduce IL-8 but can increase it at high doses, unlike heat-killed LGG.

Area of Science:

  • Gastroenterology
  • Immunology
  • Microbiology

Background:

  • Probiotic bacteria, like Lactobacillus rhamnosus GG (LGG), may have altered activity due to the gastrointestinal environment or co-administration with antibiotics.
  • The impact of these factors on probiotic anti-inflammatory effects requires further investigation.

Purpose of the Study:

  • To investigate if live or heat-killed LGG can downregulate tumor necrosis factor-alpha (TNFalpha)-induced interleukin-8 (IL-8) production in Caco-2 cells.
  • To determine if LGG modulates the nuclear factor kappaB (NFkappaB)/inhibitor of kappaB (IkappaB) pathway under various conditions.

Main Methods:

  • Caco-2 cells were treated with live or heat-killed LGG (10^4–10^10 cfu/L) with or without antibiotics and TNFalpha.
  • Interleukin-8 (IL-8) production and NFkappaB/IkappaB pathway activation were measured.

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Main Results:

  • LGG modulated TNFalpha-induced IL-8 production under all tested conditions.
  • Heat-killed LGG significantly blunted TNFalpha-induced IL-8 production.
  • High doses of live LGG increased IL-8 production, whereas heat-killed LGG showed only a slight increase.

Conclusions:

  • LGG affects the NFkappaB/IkappaB pathway, reducing TNFalpha-induced IL-8 production in Caco-2 cells.
  • Heat-killed LGG appears to be a safer alternative for ameliorating inflammation compared to live LGG at high doses due to lower inflammatory potential.