[Relationship between glomerular lesion and NF-kappaB p65 activity in rat multiple organ failure caused by zymosan]

To observe the relationship between NF-kappaB p65 activity and glomerular lesion in rat multiple organ failure (MOF) caused by zymosan.

Twenty female Wistar rats aged 6-8 weeks were divided randomly into two groups, ten each group. 24 hours after MOF model was induced successfully by intraperitoneal injection of zymosan-paraffin oil suspension, the rats were sacrificed and the functions of liver and kidney as well as blood gas were analysed. At the meaning time, the pathological change in renal tissues was examined by H.E, PAS, PASM and Masson staining, whereas the NF-kappaB p65 activity in glomeruli was detected by immunohistochemical staining and ELISA.

The levels of PO(2) and pH in blood of MOF model group were significantly lower than those of control group, while the levels of serum ALT and Cr were quite to the contrary. Pathological examination showed that in MOF model rats, there were notable proliferation of glomeruli, partial sclerotic glomeruli and a large number of infiltrating inflammatory cells in glomeruli and periglomeruli. Besides, the activity of NF-kappaB p65 in rat glomeruli of MOF model group was significantly higher than that of control group(P<0.01), and the increased activity of NF-kappaB p65 was positively corelated to the cell number in glomeruli, and area density of basement membrane as well as mesangial area, but negatively correlated to the area density of capillary loop.

The increase in the activity of NF-kappaB p65 may play an important role in glomerular lesions of MOF model rats caused by zymosan.