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Updated: Aug 17, 2026
Tractable In Vivo Reprogramming of Tumor Cells to Type 1 Conventional Dendritic Cell-like Cells
Published on: August 1, 2025
[Induction of specific immunity against tumor endothelial cells by dendritic cells in vitro]
Bang-de Xiang1, Ming-de Lu, Qing Tang
1Department of Hepatobiliary Surgery, The First Affiliated Hospital, SUN Yat-Sen University, Guangzhou 510080, China.
Aim:
To study the specific cytotoxicity of cytotoxic T lymphocytes(CTLs) induced by dendritic cells(DCs) loaded with tumor-derived endothelial cells (Td-ECs) antigen.
Methods:
Td-ECs were generated from human umbilical vein endothelial cells (HUVECs) by induction with cultured supernatants of tumor cells. The expression of tumor endothelial marker(TEM) was detected by RT-PCR. Human peripheral blood monocyte-derived DCs were loaded with Td-EC freeze-thawing antigen, and the autologous stimulation capacity of DCs was determined via mixed lymphocytes reaction employing MTS colorimetry. Specific cytolytic activity of CTLs induced by DCs was assessed by lactate dehydrogenase(LDH) assay.
Results:
TEM1 and TEM8 were detected only on Td-ECs. DCs loaded with Td-EC antigen stimulated lymphocytes proliferation significantly. CTLs induced by DCs loaded with Td-EC antigen showed higher cytolytic activity against Td-EC than that of the control groups (33% vs 14% at E/T ratio 20, 27% vs 10% at E/T ratio 10).
Conclusion:
DC loaded with Td-EC antigen can effectively induce specific CTLs against Td-ECs in vitro.
