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Updated: Aug 17, 2026
Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages
Published on: April 20, 2018
Reduced interleukin-8 response to Streptococcus pneumoniae by alveolar macrophages from adults with HIV/AIDS
Stephen B Gordon1, Elizabeth R Jarman, Stonard Kanyanda
1Malawi-Liverpool-Wellcome Trust Research Programme, Universities of Malawi and Liverpool, Malawi. sbgordon@liverpool.ac.uk
Background:
HIV-infected adults are highly susceptible to pneumococcal disease.
Objective:
To examine if alveolar macrophages from HIV-infected subjects exhibited a failure of cytokine production in response to Streptococcus pneumoniae in vitro.
Design:
Case-control comparison of alveolar macrophages from 11 HIV-infected and 13 non-infected adults.
Methods:
Type 1 opsonized S. pneumoniae were used to challenge the alveolar macrophages in vitro. Cell supernatant fluid was collected from unstimulated cells, and cells challenged with bacteria for 0, 6, 12 and 24 h. Cytokine production (interleukins 1beta, 6 and 8) was measured in all fluids using an enzyme-linked immunosorbent assay.
Results:
All the cytokines tested increased over time in both HIV-infected and uninfected subjects. Interleukin-8 release was significantly lower in HIV-infected than in non-HIV-infected subjects (P = 0.02).
Conclusion:
Reduced interleukin-8 production may result in decreased neutrophil recruitment, and hence increased susceptibility to pneumococcal infection in HIV-infected subjects.
