Related Experiment Videos
Method to identify specific alleles of a Phanerochaete chrysosporium gene encoding lignin peroxidase
J Gaskell1, A Vanden Wymelenberg, P Stewart
1Institute for Microbial and Biochemical Technology, USDA Forest Service, Madison, Wisconsin 53705.
Applied and Environmental Microbiology
|April 1, 1992
Abstract:
A method to identify and differentiate allelic variants of the gene encoding lignin peroxidase isozyme H8 is presented. The strategy involves amplifying a variable region of the gene's carboxy terminus by use of the polymerase chain reaction and then probing with allele-specific oligonucleotides.