Related Experiment Video
Updated: Aug 17, 2026

Standardization of a Novel Semi-Automatic Software for Neurite Outgrowth Measurement
Published on: August 9, 2024
A novel function of transcription factor alpha-Pal/NRF-1: increasing neurite outgrowth
Wen-Teng Chang1, Hsiun-ing Chen, Rong-Jing Chiou
1Institute of Basic Medical Sciences, National Cheng Kung University College of Medicine, Tainan 701, Taiwan.
Abstract:
Alpha-Pal/NRF-1 is a critical regulator of the promoter of human IAP/CD47 gene, a gene related to memory formation in rodents. However, its function in neurons was unknown. We found that stable or transient expression of full-length alpha-Pal/NRF-1 in human neuroblastoma IMR-32 cells significantly induced neurite outgrowth and increased the length of neurites both in medium containing 10% fetal bovine serum and in serum-free medium. In contrast, the dominant-negative mutant of alpha-Pal/NRF-1 inhibited the induction and extension of neurites. Ectopic expression of full-length alpha-Pal/NRF-1 also increased the induction of neurite outgrowth in primary mouse cortical neurons. The IAP antisense cDNA significantly inhibited the increase of neurite outgrowth by alpha-Pal/NRF-1. These findings indicate that a novel function of alpha-Pal/NRF-1 is to regulate neuronal differentiation, and that this function is mediated partly via its downstream IAP gene.
Related Concept Videos
NF-kB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-κB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Enzyme-linked Receptors
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Nitric Oxide Signaling Pathway
MAPK Signaling Cascades
Regulation of the Unfolded Protein Response
