Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Messenger RNA processing sites in Trypanosoma brucei.

Corinna Benz1, Daniel Nilsson, Björn Andersson

  • 1Zentrum für Molekulare Biologie, Im Neuenheimer Feld 282, D69120 Heidelberg, Germany.

Molecular and Biochemical Parasitology
|July 5, 2005
PubMed
Summary

Researchers identified key sequence rules for trypanosome mRNA processing. This enables prediction of trans splicing and polyadenylation sites in protein-coding genes, advancing understanding of Kinetoplastid gene expression.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bayesian fine-mapping pinpoints candidate genes and pleiotropic loci of production traits from a chicken backcrossing scheme.

BMC genomics·2026
Same author

Genetically Determined Levels of Inflammation-Related Proteins and Functional Outcome After Ischemic Stroke: A Mendelian Randomization Study.

Journal of stroke·2026
Same author

Predicting post-stroke functional outcome using explainable machine learning and integrated data.

Scientific reports·2026
Same author

Integrated Methylome-Transcriptome Analysis Reveals Epigenomic Remodeling and Rho GTPase-Linked Immune-Epithelial Crosstalk in Atopic Dermatitis.

Allergy·2026
Same author

Deep learning can automate chicken tibia-breaking strength quantification to improve animal welfare.

Poultry science·2026
Same author

Elevated acute-phase plasma levels of S100A12 [EN-RAGE] are associated with vascular recurrence after ischemic stroke.

European stroke journal·2026

Area of Science:

  • Molecular Biology
  • Genetics
  • Parasitology

Background:

  • Kinetoplastids transcribe protein-coding genes polycistronically using RNA polymerase II.
  • Mature messenger RNAs (mRNAs) result from 5' trans splicing and 3' polyadenylation of precursor transcripts.
  • Trans splicing typically occurs at an AG dinucleotide downstream of a polypyrimidine tract, coupled with polyadenylation.

Purpose of the Study:

  • To precisely define sequence requirements for mRNA processing in trypanosomes.
  • To develop a predictive algorithm for trans splicing and polyadenylation sites.
  • To enhance understanding of gene expression regulation in Kinetoplastids.

Main Methods:

  • Analysis of available Trypanosoma brucei complementary DNAs (cDNAs).
  • Identification and mapping of splicing and polyadenylation sites.

Related Experiment Videos

  • Development and calibration of a grammar model with distance constraints.

    • Main Results:

    • Trans splicing generally occurs at the first AG after an 8-25 nt polypyrimidine tract, yielding median 5'-untranslated regions (5'-UTRs) of 68 nt.
    • Polyadenylation typically occurs 80-140 nt downstream of the polypyrimidine tract, often at A-rich sites.
    • A predictive algorithm was developed, capable of identifying these sites in most trypanosome protein-coding genes.

    Conclusions:

    • Established sequence parameters for trypanosome mRNA processing.
    • Developed a computational tool for predicting mRNA processing sites in the trypanosome genome.
    • The findings facilitate a deeper understanding of gene expression mechanisms in Kinetoplastids.