Related Experiment Video
Updated: Aug 17, 2026
Identifying, Diagnosing, and Grading Malignant Peripheral Nerve Sheath Tumors in Genetically Engineered Mouse Models
Published on: May 17, 2024
Expression of PDE11A in normal and malignant human tissues
Michael R D'Andrea1, Yuhong Qiu, Donna Haynes-Johnson
1Drug Discovery, Johnson & Johnson Pharmaceutical Research & Development, LLC, 1000 Route 202, Raritan, NJ 08869, USA.
Abstract:
Cyclic nucleotide phosphodiesterase 11A (PDE11A) is the newest member in the PDE family. Although the tissue distribution of PDE11A mRNA has been shown, its protein expression pattern has not been well studied. The goal of this report is to investigate the distribution of PDE11A proteins in a wide range of normal and malignant human tissues. We utilized a polyclonal antibody that recognized all four PDE11A isoforms. Its specificity was demonstrated by Western blot analysis on a recombinant human PDE11A protein and native PDE11A proteins in various human tissues. Immunohistochemistry showed that PDE11A is widely expressed. Various degrees of immunoreactivity were observed in the epithelial cells, endothelial cells, and smooth muscle cells of all tissues examined. The highest expression was in the epithelial, endothelial, and smooth muscle cells of the prostate, Leydig, and spermatogenic cells of the testis, the tubule epithelial cells in the kidney, the epithelial and endothelial cells in the adrenal, the epithelial cells and macrophages in the colon, and the epidermis in the skin. Furthermore, PDE11A expression was also detected in several human carcinomas. Our results suggest that PDE11A might be involved in multiple physiological processes in various organs via its ability to modulate intracellular cAMP and cGMP levels.
Insights
Cyclic nucleotide phosphodiesterase 11A (PDE11A) protein is widely expressed in normal human tissues and carcinomas. This broad distribution suggests PDE11A
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- Cyclic nucleotide phosphodiesterase 11A (PDE11A) is a recently identified member of the phosphodiesterase enzyme family.
- While PDE11A mRNA distribution is known, its protein expression patterns across human tissues remain largely uncharacterized.
Purpose of the Study:
- To investigate the protein expression pattern of PDE11A in a diverse array of normal and malignant human tissues.
- To determine the cellular localization and distribution of PDE11A proteins.
Main Methods:
- Utilized a validated polyclonal antibody targeting all four PDE11A isoforms.
- Confirmed antibody specificity via Western blot analysis using recombinant and native PDE11A proteins.
- Employed immunohistochemistry to map PDE11A protein expression across various human tissues.
Main Results:
- PDE11A protein is widely expressed in epithelial, endothelial, and smooth muscle cells across all examined normal tissues.
- Highest PDE11A expression observed in prostate, testis, kidney, adrenal gland, colon, and skin.
- PDE11A expression was also detected in several types of human carcinomas.
Conclusions:
- PDE11A protein exhibits a broad tissue distribution in humans, extending to malignant tissues.
- The widespread expression suggests a significant role for PDE11A in multiple physiological processes.
- PDE11A likely influences intracellular cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) levels in various organs.
