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Expression of PDE11A in normal and malignant human tissues
Michael R D'Andrea1, Yuhong Qiu, Donna Haynes-Johnson
1Drug Discovery, Johnson & Johnson Pharmaceutical Research & Development, LLC, 1000 Route 202, Raritan, NJ 08869, USA.
Summary
Cyclic nucleotide phosphodiesterase 11A (PDE11A) protein is widely expressed in normal human tissues and carcinomas. This broad distribution suggests PDE11A
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- Cyclic nucleotide phosphodiesterase 11A (PDE11A) is a recently identified member of the phosphodiesterase enzyme family.
- While PDE11A mRNA distribution is known, its protein expression patterns across human tissues remain largely uncharacterized.
Purpose of the Study:
- To investigate the protein expression pattern of PDE11A in a diverse array of normal and malignant human tissues.
- To determine the cellular localization and distribution of PDE11A proteins.
Main Methods:
- Utilized a validated polyclonal antibody targeting all four PDE11A isoforms.
- Confirmed antibody specificity via Western blot analysis using recombinant and native PDE11A proteins.
- Employed immunohistochemistry to map PDE11A protein expression across various human tissues.
Main Results:
- PDE11A protein is widely expressed in epithelial, endothelial, and smooth muscle cells across all examined normal tissues.
- Highest PDE11A expression observed in prostate, testis, kidney, adrenal gland, colon, and skin.
- PDE11A expression was also detected in several types of human carcinomas.
Conclusions:
- PDE11A protein exhibits a broad tissue distribution in humans, extending to malignant tissues.
- The widespread expression suggests a significant role for PDE11A in multiple physiological processes.
- PDE11A likely influences intracellular cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) levels in various organs.