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Mechanisms underlying the effects of LPS and activation-induced cytidine deaminase on IgA isotype expression

Seok-Rae Park1, Hyun-A Kim, Sung-Ki Chun

  • 1Department of Microbiology, College of Natural Sciences, Kangwon National University, Chunchon 200-701, Korea.

Molecules and Cells
|July 5, 2005
PubMed

Activation-induced cytidine deaminase (AID) is needed for Ig class switch recombination (CSR). We explored the effect of LPS on the expression of AID during B cell differentiation, and the role of AID in IgA isotype expression. In normal spleen B cells, LPS increased AID transcription up to 48 h post-stimulation, i.e. around the time of Ig CSR. TGF-b1 and AID were required for IgA expression, and LPS contributed to TGFb1-induced IgA production largely by inducing AID. Interestingly, LPS repressed AID transcription in sIgA+ B cells but still stimulated IgA production mainly by increasing the rate of IgA secretion. Our data indicate that LPS contributes to TGFb1-induced IgA isotype expression in at least two ways: by stimulating AID transcription before CSR and by enhancing the IgA secretion rate after CSR.

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