Related Experiment Video
Updated: Jul 23, 2026
Spheroid Assay to Measure TGF-β-induced Invasion
Published on: November 16, 2011
Expression of mRNA coding for TGF-beta and its receptors in irradiated human breast carcinoma MCF-7 cells differing
Inna Chorna1, Oleksandr Fedorenko, Leonid Datsyuk
1Institute of Cell Biology, NAS of Ukraine, Lviv 79005, Ukraine.
Aim:
The effect of X-irradiation on the expression of mRNAs coding for transforming growth factor beta (TGF-beta) and its receptors was compared in human breast carcinoma MCF-7 cells differing in their sensitivity to doxorubicin.
Methods:
Semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was used for the estimation of mRNAs expression for TGF-beta1, TGF-beta2 and TGF-beta type I and II receptors in the unirradiated and irradiated cells of two sublines of MCF-7 cells--sensitive (MCF-7(wt)) and resistant (MCF-7(DOX/R)) to doxorubicin.
Results:
X-irradiation caused an increase in TGF-beta1 coding for mRNA expression in both breast cancer cell lines tested and an elevation of TGF-beta2 mRNA expression in doxorubicin-resistant subline. The down-regulation of TGF-beta type I and II receptors in the irradiated MCF-7(DOX/R) cells was revealed at the transcriptional level.
Conclusions:
The results of our study show that the development of refractoriness of used tumor cells to anti-cancer drug doxorubicin, at least partly, can be caused by the impairment in TGF-beta signaling pathway.
