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Insertional mutagenesis in mice: new perspectives and tools.
Corey M Carlson1, David A Largaespada
1Department of Genetics, Cell Biology, and Development, University of Minnesota Cancer Center, Minneapolis, Minnesota 55455, USA.
Nature Reviews. Genetics
|July 5, 2005
Summary
New mouse models advance functional genomics through insertional mutagenesis. These methods enable faster genome annotation by creating and prescreening mutations in embryonic stem cells and using transposable elements.
Area of Science:
- Genomics
- Molecular Biology
- Genetics
Background:
- Insertional mutagenesis is a foundational technique in functional genomics.
- Advances in mouse models have refined genome-wide and phenotype-driven insertional mutagenesis screens.
Purpose of the Study:
- To highlight advancements in insertional mutagenesis for mouse functional genomics.
- To discuss the impact of new technologies on mouse genome annotation.
Main Methods:
- Utilizing improved vectors and methodologies for genome-wide insertional mutagenesis screens.
- Generating homozygous diploid mutations in mouse embryonic stem cells for prescreening.
- Employing active transposable elements for in vivo forward insertional mutagenesis screens.
Main Results:
- Enhanced ease and efficiency in conducting insertional mutagenesis screens in mice.
- Ability to prescreen for null phenotypes in embryonic stem cells before in vivo studies.
- Development of transposable elements as genetic tools for in vivo screens.
Conclusions:
- New insertional mutagenesis technologies significantly accelerate functional annotation of the mouse genome.
- These methods improve the speed and ease of genetic research in mice.