Related Experiment Video
Updated: Aug 17, 2026
Live Imaging to Quantify Cellular Radiosensitivity in Patient-Derived Tumor Organoids
Published on: April 5, 2024
Comparative study on radiosensitivity of various tumor cells and human normal liver cells
Jian-She Yang1, Wen-Jian Li, Guang-Ming Zhou
1Radiobiology Laboratory, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, Gansu Province, China. tuyangjs@impcas.ac.cnit
Aim:
To investigate the radiation response of various human tumor cells and normal liver cells.
Methods:
Cell lines of human hepatoma cells (SMMC-7721), liver cells (L02), melanoma cells (A375) and cervical tumor (HeLa) were irradiated with (60)Co gamma-rays. Cell survive was documented by a colony assay. Chromatid breaks were measured by counting the number of chromatid breaks and isochromatid breaks immediately after prematurely chromosome condensed by Calyculin-A.
Results:
Linear quadratic survival curve was observed in all of four cell lines, and dose-dependent increase in radiation-induced chromatid and isochromatid breaks were observed in GB2B phase. Among these four cell lines, A375 was most sensitive to radiation, while, L02 had the lowest radiosensitivity. For normal liver cells, chromatid breaks were easy to be repaired, isochromatid breaks were difficult to be repaired.
Conclusion:
The results suggest that the gamma-rays induced chromatid breaks can be possibly used as a good predictor of radiosensitivity, also, unrejoined isochromatid breaks probably tightly related with cell cancerization.
