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Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A EYFP-CENP-A
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S(O)-pixyl protecting group as efficient mass-tag.

Pablo L Bernad1, Safraz Khan, Vladimir A Korshun

  • 1Tridend Technologies (a Division of OGT), Hirsch Building, Begbroke Business & Science Park, Sandy Lane, Yarnton, Oxford UK OX5 1PF.

Chemical Communications (Cambridge, England)
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Summary

Novel trityl tags with adjustable stability were designed and prepared. These compounds show efficiency as protecting groups and mass spectrometry (MS) analytes.

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Area of Science:

  • Organic Chemistry
  • Synthetic Chemistry

Background:

  • Protecting groups are crucial in organic synthesis.
  • Mass spectrometry (MS) is a key analytical technique.

Purpose of the Study:

  • To design and synthesize novel trityl tags.
  • To evaluate their stability and applications as protecting groups and MS analytes.

Main Methods:

  • Chemical synthesis of trityl derivatives.
  • Stability studies under various conditions.
  • Application testing as protecting groups.
  • Analysis as MS analytes.

Main Results:

  • Successful design and preparation of new trityl tags.
  • Demonstrated adjustable stability of the novel tags.
  • Efficient performance as protecting groups.
  • Effective utility as MS analytes.

Conclusions:

  • The developed trityl tags offer tunable stability.
  • These tags present versatile applications in synthesis and analysis.