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Related Experiment Videos

Transcriptome expression profiles in prenatal pigs in relation to myogenesis.

Marinus F W Te Pas1, Agnes A W De Wit, Jan Priem

  • 1Division of Animal Resource Development, Wageningen University and Research, Animal Sciences Group ID-Lelystad, Animal Genomics Group, P.O. Box 65, 8200 AB Lelystad, The Netherlands. marinus.tepas@wur.nl

Journal of Muscle Research and Cell Motility
|July 7, 2005
PubMed
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Prenatal pig muscle development involves complex gene expression changes. Key myogenic differentiation genes peak around day 35, with proliferation preceding fiber formation.

Area of Science:

  • Animal Science
  • Developmental Biology
  • Molecular Genetics

Background:

  • Pigs are valuable models for studying muscle growth due to selective breeding for efficient muscle development.
  • Understanding myogenesis, the process of muscle fiber formation, is crucial for livestock productivity and developmental biology.

Purpose of the Study:

  • To investigate the temporal gene expression profiles related to myogenesis, muscle structure, and energy metabolism in prenatal pigs.
  • To identify key regulatory periods and gene expression dynamics during muscle fiber development from 14 to 91 days of gestation.

Main Methods:

  • Utilized microarray technology to analyze gene expression profiles in prenatal pig muscle tissue.
  • Quantified gene activation and repression by counting detectable signal spots.

Related Experiment Videos

  • Examined expression levels of genes involved in myogenesis, muscle structure, and energy metabolism.

    • Main Results:

    • Upregulation of muscle structural protein genes increased steadily throughout gestation.
    • Genes influencing myogenic differentiation (both stimulatory and inhibitory) peaked around day 35.
    • Myoblast proliferation genes peaked before day 35, preceding differentiation.
    • Energy metabolism genes (glycolysis, ATP) were lowest during myoblast fusion (days 35-63).

    Conclusions:

    • Myogenic differentiation depends on the balance between activating and inhibiting gene expression, not just expression levels.
    • Muscle structural development is a continuous process throughout prenatal development.
    • Energy metabolism is downregulated during the critical phase of myoblast fusion into multinucleated muscle fibers.