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Extended-spectrum-beta-lactamase-producing Enterobacteriaceae in Yaounde, Cameroon
Joseph Gangoué-Piéboji1, Branka Bedenic, Sinata Koulla-Shiro
1Laboratory of General Biology, Faculty of Science, University of Yaoundé I, P.O. Box 812 Yaounde, Cameroon. jgangoue@yahoo.fr
Journal of Clinical Microbiology
|July 8, 2005
Summary
Extended-spectrum beta-lactamases (ESBLs) were identified in 12% of Enterobacteriaceae strains in Cameroon. The study describes the first detection of the ESBL SHV-12 in the country, highlighting a significant public health concern.
Area of Science:
- Microbiology
- Infectious Diseases
- Molecular Biology
Background:
- Extended-spectrum beta-lactamases (ESBLs) confer resistance to key antibiotics.
- Prevalence data for ESBL-producing Enterobacteriaceae in Cameroon were previously unavailable.
- ESBLs pose a growing global threat to effective antimicrobial therapy.
Purpose of the Study:
- To determine the prevalence of ESBL-producing Enterobacteriaceae in Cameroon.
- To characterize the ESBLs identified in clinical isolates.
- To report the first description of ESBL SHV-12 in Cameroon.
Main Methods:
- Isolation and screening of 259 Enterobacteriaceae strains from Yaounde Central Hospital (1995-1998).
- Double-disk (DD) synergy test for ESBL detection.
- Conjugation experiments to transfer resistance genes and DNA sequencing for beta-lactamase identification.
Main Results:
- Thirty-one (12%) of the isolates were ESBL-positive.
- Resistance genes from 12 strains were successfully transferred via conjugation.
- DNA sequencing identified the beta-lactamase as SHV-12 in all 12 transconjugants.
Conclusions:
- The ESBL SHV-12 is prevalent in Enterobacteriaceae in Cameroon.
- This finding represents the first documented occurrence of ESBL SHV-12 in Cameroon.
- The study underscores the need for surveillance of antimicrobial resistance in the region.