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Updated: Aug 17, 2026

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
Published on: February 17, 2017
Enhanced trehalose production improves growth of Escherichia coli under osmotic stress
J E Purvis1, L P Yomano, L O Ingram
1Dept. of Microbiology and Cell Science, Bldg. 981 Museum Road, IFAS, P.O. Box 110700, University of Florida, Gainesville, FL 32611, USA.
Abstract:
The biosynthesis of trehalose has been previously shown to serve as an important osmoprotectant and stress protectant in Escherichia coli. Our results indicate that overproduction of trehalose (integrated lacI-Ptac-otsBA) above the level produced by the native regulatory system can be used to increase the growth of E. coli in M9-2% glucose medium at 37 degrees C to 41 degrees C and to increase growth at 37 degrees C in the presence of a variety of osmotic-stress agents (hexose sugars, inorganic salts, and pyruvate). Smaller improvements were noted with xylose and some fermentation products (ethanol and pyruvate). Based on these results, overproduction of trehalose may be a useful trait to include in biocatalysts engineered for commodity chemicals.
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