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Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
Published on: May 16, 2017
High-level expression and secretion of methyl parathion hydrolase in Bacillus subtilis WB800
Xiao-Zhou Zhang1, Zhong-Li Cui, Qing Hong
1Department of Microbiology, MOA Key Laboratory of Microbiological Engineering of Agricultural Environment, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
Abstract:
The methyl parathion hydrolase (MPH)-encoding gene mpd was placed under the control of the P43 promoter and Bacillus subtilis nprB signal peptide-encoding sequence. High-level expression and secretion of mature, authentic, and stable MPH were achieved using the protease-deficient strain B. subtilis WB800 as the host.
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