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Updated: Aug 17, 2026
From Voxels to Knowledge: A Practical Guide to the Segmentation of Complex Electron Microscopy 3D-Data
Published on: August 13, 2014
Technical report on semiautomatic segmentation using the Adobe Photoshop
Jin Seo Park1, Min Suk Chung, Sung Bae Hwang
1Department of Anatomy, Ajou University School of Medicine, 5 Woncheon-dong, Yeongtong-gu, Suwon, 443-749, South Korea.
Abstract:
The purpose of this research is to enable users to semiautomatically segment the anatomical structures in magnetic resonance images (MRIs), computerized tomographs (CTs), and other medical images on a personal computer. The segmented images are used for making 3D images, which are helpful to medical education and research. To achieve this purpose, the following trials were performed. The entire body of a volunteer was scanned to make 557 MRIs. On Adobe Photoshop, contours of 19 anatomical structures in the MRIs were semiautomatically drawn using MAGNETIC LASSO TOOL and manually corrected using either LASSO TOOL or DIRECT SELECTION TOOL to make 557 segmented images. In a similar manner, 13 anatomical structures in 8,590 anatomical images were segmented. Proper segmentation was verified by making 3D images from the segmented images. Semiautomatic segmentation using Adobe Photoshop is expected to be widely used for segmentation of anatomical structures in various medical images.
