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Updated: Aug 17, 2026

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies
Published on: November 18, 2022
Dynamics of multiple trapping by a single-beam laser tweezer
Daniel S Kaputa1, Andrey N Kuzmin, Aliaksandr V Kachynski
1The Institute for Lasers, Photonics, and Biophotonics, State University of New York at Buffalo, 458 Natural Sciences Complex, Buffalo, New York 14260-3000, USA. dskaputa@buffalo.edu
Abstract:
A multiple-trap single-beam scanning laser tweezer system was developed and characterized. Different stationary and mobile multiple-trap modes were generated for polystyrene beads in a water environment. Trapping efficiency and stability were investigated for several dynamic parameters such as transition time between the sites, waiting time on a single site, number of trapping sites, and IR laser power. Optimal parameters for efficient generation of complex arrays and matrices were determined. We demonstrate an example of a single laser beam multiple-trap application by measuring the trap's stiffness in water for our laser tweezer setup.

