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Related Experiment Videos

High-throughput purification of single compounds and libraries.

Mathias Schaffrath1, Erich von Roedern, Peter Hamley

  • 1Chemistry, Aventis Pharma Deutschland GmbH, a Company of the Sanofi-Aventis Group, 65926 Frankfurt am Main, Germany.

Journal of Combinatorial Chemistry
|July 12, 2005
PubMed
Summary

Medicinal chemistry requires efficient compound purification. New automated chromatography units enhance high-throughput purification, processing up to 1000 compounds weekly for drug discovery.

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Area of Science:

  • Medicinal Chemistry
  • Chemical Engineering
  • Laboratory Automation

Background:

  • Increasing productivity in medicinal chemistry necessitates advancements in automated synthesis and purification technologies for compound libraries.
  • Traditional purification methods struggle to keep pace with high-throughput compound library production, posing a significant organizational challenge.

Purpose of the Study:

  • To establish integrated, full-service chromatography units to address the challenges in high-throughput compound purification.
  • To combine high-throughput purification capabilities with meticulous attention to detail characteristic of dedicated services.

Main Methods:

  • Implementation of three automated chromatography units featuring normal-phase and reversed-phase chromatography.
  • Integration of robotic fraction pooling, reformatting, bottling, automated solvent handling, and high-capacity freeze-drying.

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  • Utilization of an electronic sample registration and tracking system to manage work processes.

    • Main Results:

    • A final purification laboratory capable of processing up to 1000 compounds per week (5-300 mg scale).
    • Two intermediate purification units handling 100 samples per week (0.3-100 g scale).
    • Successful integration of diverse automation technologies for streamlined purification workflows.

    Conclusions:

    • The newly established chromatography units effectively meet the demand for high-throughput compound purification in medicinal chemistry.
    • The automated systems enhance efficiency and precision, supporting large-scale compound library production.
    • This integrated approach represents a significant advancement in purification technology for drug discovery efforts.