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Importance of quantitative PCR primer location for short interfering RNA efficacy determination

Allan R Shepard1, Nasreen Jacobson, Abbot F Clark

  • 1Glaucoma Research, Alcon Research Ltd., Fort Worth, TX 76134, USA. allan.shepard@alconlabs.com

Analytical Biochemistry
|July 12, 2005
PubMed
Abstract

No abstract available in PubMed .

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...