Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Understanding enzyme action on immobilised substrates.

Peter J Halling¹, Rein V Ulijn, Sabine L Flitsch

¹Department of Pure & Applied Chemistry, University of Strathclyde, Glasgow G1 1XW, UK. p.j.halling@strath.ac.uk

Current Opinion in Biotechnology

|July 12, 2005

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Water-Mediated Reconfigurable Topology and Mechanics in Porous Peptide Materials.

Matter·2026

Same author

Metabolic thermodynamics: pertinent reference state and energy potentials.

The FEBS journal·2026

Same author

Backbone-Constrained Tripeptides Enable Sequence Control of Solid-State Dynamics.

Angewandte Chemie (International ed. in English)·2026

Same author

Directed discovery of high-loading nanoaggregates enabled by drug-matched oligo-peptide excipients.

Chem·2026

Same author

Selective N-Terminal Modification of Peptides and Proteins Using Fatty Acyl Phosphates.

Angewandte Chemie (International ed. in English)·2026

Same author

Peptide Coacervates Can Protect Sequestered Oligonucleotides from Nucleases and Release Them for Transcription and Translation.

Biomacromolecules·2025

Same journal

Microbial C1 assimilation pathways for chemical synthesis: from native metabolism to synthetic design.

Current opinion in biotechnology·2026

Same journal

Medicinal plants fermentation: current knowledge and perspectives.

Current opinion in biotechnology·2026

Same journal

Fermented foods: lessons learned from metagenomics.

Current opinion in biotechnology·2026

Same journal

Microfluidic platforms for the transient transfection of mammalian cells: recent developments and challenges.

Current opinion in biotechnology·2026

Same journal

Harvesting insights from recent advances in yeast genomics for predictable and precision wine fermentation.

Current opinion in biotechnology·2026

Same journal

Minimal enzyme cascades for the aromatic-to-aromatic upgrading of lignin monomers.

Current opinion in biotechnology·2026

See all related articles

Solid-supported chemistry enables automated synthesis and screening by immobilizing substrates on surfaces. New methods analyze enzyme action on these immobilized substrates, revealing altered reaction kinetics and thermodynamics.

Area of Science:

Biochemistry
Chemical Engineering
Materials Science

Background:

Solid-supported chemistry is gaining traction for automated synthesis and screening.
Surface-immobilized substrates are crucial for studying enzyme behavior.

Purpose of the Study:

To review methods for analyzing enzyme action on solid-supported substrates.
To discuss the impact of immobilization on enzyme kinetics and thermodynamics.

Main Methods:

Fluorescence measurements
MALDI-TOF mass spectrometry
Quartz crystal microbalances
Spatial resolution techniques for single beads

Main Results:

Novel methods allow direct monitoring of enzyme activity on immobilized substrates.

Related Experiment Videos

Enzyme accessibility within solid supports is increasingly understood.

Surface immobilization can significantly shift reaction equilibrium compared to solution-phase reactions.

Conclusions:

Solid-supported chemistry offers unique advantages for enzyme studies.
Immobilization influences enzyme kinetics and thermodynamics, opening new research avenues.
Further research is needed to fully characterize modified kinetics in surface-immobilized systems.