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Initial formation of cellular intrinsic fiber cementum in developing human teeth. A light- and electron-microscopic
1Department of Oral Structural Biology, University of Zürich, Switzerland.
Cell and Tissue Research
|February 1, 1992
Summary
This study reveals how cellular intrinsic fiber cementum (CIFC) forms in developing human teeth. Cementoblasts initiate rapid cementum thickening near the root edge, contributing to tooth root development.
Area of Science:
- Biomineralization
- Dental Histology
- Cell Biology
Background:
- Cellular intrinsic fiber cementum (CIFC) is a crucial component of the periodontium.
- Understanding CIFC initiation is vital for comprehending tooth root development and repair.
Purpose of the Study:
- To elucidate the formative process and initiation of cellular intrinsic fiber cementum (CIFC) in human teeth during root development.
Main Methods:
- Electron microscopy (light and transmission electron microscopy) was used to examine 8 premolars with incompletely formed roots.
- Scanning electron microscopy was employed on 5 additional teeth after organic material removal.
- Samples were processed using Karnovsky's fixative, EDTA decalcification, osmication, and Epon embedding.
Main Results:
- CIFC initiation occurs near the advancing root edge, leading to rapid cementum thickening.
- Cementoblasts, rich in rough endoplasmic reticulum, drive both rapid and slow CIFC formation.
- The CIFC matrix contains intrinsic collagen fibrils, lacks Sharpey's fibers, and initially intermingles with unmineralized dentinal collagen.
Conclusions:
- Cementoblasts play a key role in the formation of CIFC through appositional growth.
- The intrinsic collagen fibrils within CIFC are organized parallel to the root surface.
- Intracytoplasmic collagen fibrils in cementoblasts may be involved in fibril formation.