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Updated: Aug 17, 2026
Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids
Published on: March 17, 2016
A real-time RT-quantative(q)PCR for the detection of bovine ephemeral fever virus
Yehuda Stram1, Larisa Kuznetzova, Aviad Levin
1Virology Division, Kimron Veterinary Institute, P.O. Box 12, Beit Dagan 50250, Israel. stramy@int.gov.il
Abstract:
A quantitative reverse-transcriptase real-time PCR assay, using TaqMan chemistry, for detecting bovine ephemeral virus (BEFV) is described. Available G gene sequences of viral RNA were aligned, and primers and probes were designed to recognize the virus. To quantitate the viruses, cDNA containing the real-time amplicon was prepared with a forward primer carrying the T7 promoter sequences. Run-off transcription from the T7 promoter amplicon template was used to prepare cRNA. Ten-fold dilutions of the run-off viral transcript were used as templates for the reaction in which they served as standards to quantitate unknown viral samples. By using this system it was shown that as few as 10-100 copies of a viral genome could be detected.
