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Updated: Aug 17, 2026

Bioreactor Assembly for Continuous Culture of Complex Fecal Communities
Published on: April 25, 2025
Biofilm microbial community of a thermophilic trickling biofilter used for continuous biohydrogen production
Yeonghee Ahn1, Eun-Jung Park, You-Kwan Oh
1Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea. yahn@kaist.ac.kr
Abstract:
Molecular methods were employed to investigate the microbial community of a biofilm obtained from a thermophilic trickling biofilter reactor (TBR) that was operated long-term to produce H(2). Biomass concentration in the TBR gradually decreased as reactor bed height increased. Despite this difference in biomass concentration, samples from the bottom and middle of the TBR bed revealed similar microbial populations as determined by PCR-DGGE analysis of 16S rRNA genes. Nucleotide sequences of most DGGE bands were affiliated with the classes Clostridia and Bacilli in the phylum Firmicutes, and the most dominant bands showed a high sequence similarity to Thermoanaerobacterium thermosaccharolyticum.
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