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Updated: Aug 17, 2026
High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization
Published on: June 28, 2012
Physical map of the Azoarcus sp. strain BH72 genome based on a bacterial artificial chromosome library as a platform
Federico Battistoni1, Daniela Bartels, Olaf Kaiser
1Laboratory of General Microbiology, University Bremen, P.O. Box 33 04 40, D-28334 Bremen, Germany.
Abstract:
Azoarcus sp. strain BH72 is a Gram-negative proteobacterium of the beta subclass; it is a diazotrophic endophyte of graminaceous plants and can provide significant amounts of fixed nitrogen to its host plant Kallar grass. We aimed to obtain a physical map of the Azoarcus sp. strain BH72 chromosome to be directly used in functional analysis and as a part of an Azoarcus sp. BH72 genome project. A bacterial artificial chromosome (BAC) library was constructed and analysed. A representative physical map with a high density of marker genes was developed in which 64 aligned BAC clones covered almost the entire genome.
