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Updated: Aug 17, 2026

Assessing Respiratory Immune Responses to Haemophilus Influenzae
Published on: June 29, 2021
Variation in selected regions of blaTEM genes and promoters in Haemophilus influenzae
Stephen G Tristram1, Rebecca Hawes, Juliana Souprounov
1School of Human Life Sciences, University of Tasmania, Launceston, Tasmania 7250, Australia. Stephen.Tristram@utas.edu.au
Objectives:
To determine if the TEM beta-lactamases of Haemophilus influenzae are TEM-1 or derivatives thereof and associated exclusively with the overlapping Pa/Pb promoters.
Methods:
Single nucleotide specific PCR was used to discriminate the polymorphic nucleotides at positions 32 and 317 of the bla(TEM) genes of a collection of TEM-positive strains.
Results:
All bla(TEM) genes were found to be bla(TEM-1) or derivatives thereof and none bla(TEM-2). The bla(TEM) genes were associated with the P3 promoter, the Pa/Pb promoters or a novel promoter produced as a result of a 135 bp deletion and a G162T substitution.
Conclusions:
The genetic features of bla(TEM) genes in H. influenzae are different from those in Enterobacteriaceae and more variable than previously recognized.
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