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Related Experiment Videos

Pressurised solvent extraction for organotin speciation in vegetable matrices.

Christophe Marcic1, Gaëtane Lespes, Martine Potin-Gautier

  • 1Group of Analytical Chemistry-LCABIE, UMR-CNRS 5034, Université de Pau et des Pays de l'Adour, Av. de l'Université, BP 1155, 64013, Pau, France. christophe.marcic@univ-pau.fr

Analytical and Bioanalytical Chemistry
|July 12, 2005
PubMed
Summary

Pressurised solvent extraction (PSE) offers an efficient method for detecting organotin compounds (OTCs) in vegetables. This optimized technique provides reliable results for food safety monitoring, with shorter extraction times than traditional methods.

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Area of Science:

  • Environmental Chemistry
  • Analytical Chemistry
  • Food Safety

Background:

  • Organotin compounds (OTCs) are widespread environmental contaminants due to extensive industrial use.
  • Monitoring OTCs in vegetables is crucial for food safety, yet speciation studies in plants are limited.
  • Trisubstituted OTCs, the most toxic tin species, require accurate analytical methods for detection.

Purpose of the Study:

  • To evaluate and optimize a pressurised solvent extraction (PSE) procedure for quantifying organotin content in vegetables.
  • To compare the optimized PSE method with conventional solid/liquid extraction (SLE) for efficiency and accuracy.
  • To assess the preservation of organotin speciation during the extraction process.

Main Methods:

  • Pressurised Solvent Extraction (PSE) using methanol, ethyl acetate, or a mixture.

Related Experiment Videos

  • Optimization of PSE parameters (pressure, temperature) using experimental design methodology.
  • Comparison with Solid/Liquid Extraction (SLE) using mechanical shaking in an acidic solvent.
  • Analysis of spiked samples with four trisubstituted organotins: tributyltin (TBT), triphenyltin (TPhT), tricyclohexyltin (TcHexT), and trioctyltin (TOcT).

    • Main Results:

    • A methanol and ethyl acetate mixture proved most effective for quantitative extraction while preserving speciation.
    • Optimized PSE achieved detection limits of 1-2 ng (Sn) g⁻¹, comparable to SLE (0.1-1 ng (Sn) g⁻¹).
    • PSE demonstrated similar repeatability (2-12%) to SLE but with significantly shorter extraction times (15 min vs. 2-12 h).
    • PSE ensured satisfactory OTC preservation and repeatability, making it suitable for vegetable analysis.

    Conclusions:

    • Optimized PSE is a viable and efficient technique for monitoring organotin compounds in vegetables.
    • PSE offers a faster alternative to SLE for organotin speciation in plant materials.
    • The developed method contributes to improved food safety by enabling reliable OTC detection in commonly consumed vegetables.