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Updated: Aug 17, 2026

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems
Published on: February 10, 2020
Changes in carotenoid content and distribution in living plant tissue can be observed and mapped in situ using
Rafal Baranski1, Malgorzata Baranska, Hartwig Schulz
1Institute of Horticultural Crops, Federal Centre for Breeding Research on Cultivated Plants (BAZ), Neuer Weg 22-23, 06484 Quedlinburg, Germany.
Abstract:
Near-infrared (NIR) excited Fourier transform (FT) Raman spectroscopy has been applied for in situ analysis of carotenoids in living plant samples. Pelargonium x hortorum leaf has been mapped using a Raman mapping technique to illustrate heterogeneous distribution of carotenoids. Mapping has also been employed for visualization of carotenoid changes induced by abiotic and biotic stress. In a tomato (Lycopersicon esculentum Mill.) fruit, inhibition of lycopene biosynthesis and accumulation of beta-carotene are demonstrated in tissue affected by sunscald physiological disorder. Raman map of diseased sugarbeet (Beta vulgaris L.) leaf shows a local carotenoid decline at infection site while the carotenoid accumulation is evident in parsley (Petroselinum crispum Mill. Nym.) as a response to Septoria petroselini infestation. Additionally, occurrence of lutein, beta-carotene and capsanthin, and changes in their relative content during bell pepper (Capsicum annum L.) fruit ripening are described by single Raman spectra. Based on these examples, the potential application of NIR-FT-Raman spectroscopy for a non-destructive analysis of carotenoids in various living plant tissues of the size ranging from about 0.01 mm(2) to 35 cm(2) is discussed.
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