Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cloning: eight years after Dolly.

K H S Campbell1, R Alberio, I Choi

  • 1Animal Development and Biotechnology Group, Division of Animal Physiology, University of Nottingham, School of Biosciences, Sutton Bonington, Loughborough, Leics LE12 5RD, UK. keith.campell@nottingham.ac.uk

Reproduction in Domestic Animals = Zuchthygiene
|July 13, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Communicating Personal Risk Profiles of Alzheimer's Disease to Older Adults: A Pilot Trial.

The journal of prevention of Alzheimer's disease·2022
Same author

A simple and efficient method of generating HCMV pp65-specific T cells using overlapping peptides.

Acta virologica·2020
Same author

The metabolic suppressor 3-iodothyronamine enhances lipolysis in 3T3-L1 adipocytes via activation of the adenosine monophosphate-activated protein kinase/forkhead box O1 signaling pathway.

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society·2020
Same author

Second-phase validation study of an alternative developmental toxicity test using mouse embryonic stem cell-derived embryoid bodies.

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society·2020
Same author

Survival of oral mucosal melanoma according to treatment, tumour resection margin, and metastases.

The British journal of oral & maxillofacial surgery·2020
Same author

Distinctive patterns of pulmonary function change according to baseline lung volume and diffusing capacity.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2020
Same journal

Immuno-Targeting of CLRN3 and SCAMP1 as a Potential Sex Specific Marker in Bovine Spermatozoa.

Reproduction in domestic animals = Zuchthygiene·2026
Same journal

Effect of BRD0539 on Gene Editing and Mosaicism Rate in Porcine Gene Editing Embryos by CRISPR/Cas9.

Reproduction in domestic animals = Zuchthygiene·2026
Same journal

Enhancement of Ram Sperm Quality During Chilled Storage by Supplementation With Spirulina platensis Extract.

Reproduction in domestic animals = Zuchthygiene·2026
Same journal

Associations of Management Factors and Environmental Conditions With the Number of Liveborn Piglets in a Commercial Pig Farm: A Retrospective Field Study.

Reproduction in domestic animals = Zuchthygiene·2026
Same journal

Y-Sperm Enrichment Through TLR 7/8 Validated Through Molecular and Biochemical Approaches in Sahiwal Bull.

Reproduction in domestic animals = Zuchthygiene·2026
Same journal

Evaluation of Proliferative Potential and Collagen Deposition in Vitrified Canine Testicular Fragments Subjected to Different Cryoprotectant Combinations and Warming Temperatures.

Reproduction in domestic animals = Zuchthygiene·2026
See all related articles

Nuclear transfer technology has advanced significantly since Dolly the sheep, enabling transgenic animal production. However, low efficiency and developmental issues persist, requiring procedural improvements.

Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Genetics

Background:

  • Nuclear transfer (NT) technology, pioneered by Dolly the sheep, uses adult somatic cells for cloning.
  • NT has been applied across various mammalian species, utilizing diverse fetal and adult donor cell types.
  • Genetic manipulation of donor cells has led to transgenic offspring with targeted gene alterations.

Purpose of the Study:

  • To review the successes and challenges of nuclear transfer since Dolly.
  • To analyze key procedural steps and modifications impacting developmental efficiency.
  • To discuss strategies for improving NT outcomes in mammals.

Main Methods:

  • Review of published literature on mammalian nuclear transfer techniques.
  • Analysis of studies focusing on donor cell types and genetic manipulation.

Related Experiment Videos

  • Examination of modifications to NT procedures and their developmental effects.

    • Main Results:

    • Successful application of NT in numerous mammalian species.
    • Production of genetically modified (transgenic) animals via NT.
    • Persistent low efficiency and occurrence of developmental abnormalities in NT offspring.

    Conclusions:

    • Nuclear transfer technology has demonstrated broad applicability and potential for genetic engineering.
    • Significant challenges remain in improving the efficiency and safety of NT procedures.
    • Further research into optimizing NT protocols is crucial for successful application.