Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell-to-cell interactions in changed gravity: ground-based and flight experiments.

L Buravkova1, Yu Romanov, M Rykova

  • 1SSC Institute of Biomedical Problems RAS, Moscow, Russian Federation. buravkova@imbp.ru

Acta Astronautica
|July 14, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of prolonged isolation in a confined space on status of oxidative stress and prothrombogenesis: In preparation for possible future manned space expedition to Mars.

The Malaysian journal of pathology·2020
Same author

Functional changes in neutrophils and psychoneuroendocrine responses during 105 days of confinement.

Journal of applied physiology (Bethesda, Md. : 1985)·2015
Same author

520-d Isolation and confinement simulating a flight to Mars reveals heightened immune responses and alterations of leukocyte phenotype.

Brain, behavior, and immunity·2014
Same author

Blood biochemical parameters in women during long-term simulated hyperoxic diving up to 8 ATA.

Undersea & hyperbaric medicine : journal of the Undersea and Hyperbaric Medical Society, Inc·2006
Same author

Simulated hypogravity stimulates cell spreading and wound healing in cultured human vascular endothelial cells.

Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology·2003
Same author

Phagocyte system under spaceflight conditions.

Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology·2001

Cell-cell interactions in endothelial cells (ECs) and immune cells are crucial for physiological functions. Simulated and real microgravity impact cell structures but preserve critical cell-to-cell communication and immune cell functions.

Area of Science:

  • Cell biology
  • Space biology
  • Immunology

Background:

  • Cell-to-cell interactions are vital for physiological processes, influenced by mechanical and biochemical factors.
  • Mechanosensitive cells, like endothelial cells (ECs), respond to altered gravity, affecting cytoskeletal remodeling and adhesion molecule expression.
  • Understanding these changes is crucial for maintaining cellular functions in space environments.

Purpose of the Study:

  • To investigate the effects of simulated and real microgravity on cell-to-cell interactions, specifically between endothelial cells and immune cells.
  • To determine if changes in cell structure and adhesion molecules under microgravity compromise critical physiological functions.

Main Methods:

  • Cultured endothelial cells (ECs) were subjected to clinorotation to simulate microgravity.

Related Experiment Videos

  • Cocultures of endothelial monolayers with human lymphocytes and myeloleukemic cells were used to study adhesion.
  • Cytotoxic activity of natural killer (NK) cells was measured during spaceflight experiments on the International Space Station.

    • Main Results:

    • Clinorotation increased the adhesion of activated lymphocytes to endothelial monolayers but did not affect non-activated cells.
    • Immune cells in microgravity retained their ability to contact, recognize, and destroy oncogenic cells.
    • Despite alterations in cytoskeleton and adhesion molecules, cell-cell interactions remained functional.

    Conclusions:

    • Simulated and real microgravity induce changes in endothelial cells and immune cells, including cytoskeletal remodeling and altered adhesion molecule expression.
    • These microgravity-induced changes do not compromise essential cell-to-cell interactions or the cytotoxic functions of immune cells.
    • Critical physiological functions mediated by immune and endothelial cells are preserved even under altered gravity conditions.