Related Experiment Video
Updated: Aug 17, 2026
HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis
Published on: November 11, 2022
[Determination of complanatoside A in semen Astragali complanati by HPLC]
Jian-Jun Zhang1, Xing-Li Yan, Yu-Jie Zhang
1Beijing University of Chinese Traditional Medicine, Beijing 100029, China.
Objective:
To establish the determination method for complanatoside A in seeds of Astragalus complanatus.
Method:
An HPLC method has been developed to separate complanatoside A on ZORBAX EXTEND-C18 (4.6 mm x 250 mm, 5 microm) column with acetonitrile-water-phosphoric acid (20:80:0.2) as mobile phase and UV detection at 267 nm.
Result:
The good linearity of complanatoside A ranged 0.086-0.430 microg, r = 0.9999. An average recovery of 99.8% (n = 5) was obtained with a RSD of 1.0%.
Conclusion:
The established method is proved to be stability, fast, accurate and can be used for quantification of Complanatoside A in Semen Astragali Complanati.
