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Lectin arrays for profiling cell surface carbohydrate expression.

Ting Zheng1, Dora Peelen, Lloyd M Smith

  • 1Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, Wisconsin 53706-1396, USA.

Journal of the American Chemical Society
|July 14, 2005
PubMed
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We developed a new method using lectin arrays on gold surfaces to analyze cell surface carbohydrates. This technique effectively measures carbohydrate expression patterns on different cell types.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Materials Science

Background:

  • Cell surface carbohydrates play crucial roles in biological processes.
  • Analyzing these glycans is essential for understanding cell function and disease.

Purpose of the Study:

  • To develop and validate a novel lectin array strategy for analyzing cell surface carbohydrate expression.

Main Methods:

  • Fabrication of lectin arrays on gold surfaces.
  • Immobilization of lectins and assessment of their binding specificities using antibody and glycoprotein interactions.
  • Application of the array to analyze carbohydrate patterns on two mammalian cell lines.

Main Results:

  • Lectin immobilization on gold surfaces was successful.

Related Experiment Videos

  • Immobilized lectins retained their specific carbohydrate-binding activities.
  • Distinct cell surface carbohydrate expression patterns were identified in two mammalian cell lines.

    • Conclusions:

    • The developed lectin array strategy provides a robust platform for cell surface glycan analysis.
    • This method enables precise characterization of carbohydrate expression profiles in biological samples.