Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell sheet engineering: recreating tissues without biodegradable scaffolds.

Joseph Yang1, Masayuki Yamato, Chinatsu Kohno

  • 1Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan.

Biomaterials
|July 14, 2005
PubMed
Summary

Cell sheet engineering offers a novel approach to tissue regeneration, avoiding limitations of traditional scaffolds. This method uses temperature-responsive dishes for enzyme-free cell sheet harvesting, enabling scaffold-free tissue construction.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fibronectin Peptide FNIII14 Enhances Progressive Cartilage Degeneration in Osteoarthritis by Inducing Chondrocyte Apoptosis.

Current issues in molecular biology·2026
Same author

Gelatin-based cryogels seeded with exosomes enhance osteogenic activity and bone regeneration in a rabbit femoral defect model.

Journal of biomaterials applications·2026
Same author

Reducing bias and enhancing equity in AI-enabled precision nutrition: addressing measurement error across wearables, multiomics, and dietary data.

Frontiers in digital health·2026
Same author

Real-World Health Care Resource Utilization and Economic Burden Among Patients with Idiopathic Pulmonary Fibrosis in Commercially Insured and Medicare Advantage Populations in the United States.

Journal of health economics and outcomes research·2026
Same author

Safety and Feasibility of IVC Flow Modulation With the Doraya in Acute Decompensated Heart Failure.

JACC. Heart failure·2026
Same author

Real-World Economic Burden Associated with Progressive Pulmonary Fibrosis in the US: An Administrative Claims Database Analysis.

PharmacoEconomics·2026

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Conventional tissue engineering using biodegradable scaffolds faces limitations hindering progress.
  • Biodegradable scaffolds can elicit host inflammatory responses, complicating tissue regeneration.
  • Enzymatic digestion is often required for cell harvesting in traditional methods, potentially damaging cells.

Purpose of the Study:

  • To introduce and evaluate cell sheet engineering as an alternative to traditional tissue engineering methods.
  • To overcome the limitations associated with biodegradable scaffolds and enzymatic cell harvesting.
  • To demonstrate the potential of cell sheet engineering for scaffold-free tissue regeneration.

Main Methods:

  • Development of cell sheet engineering utilizing temperature-responsive culture dishes.

Related Experiment Videos

  • Harvesting of intact cell sheets through simple temperature modulation, avoiding proteolytic enzymes.
  • Application of cell sheets via direct transplantation or layering for three-dimensional tissue construction.

    • Main Results:

    • Successful harvesting of intact cell sheets without enzymatic treatment.
    • Demonstration of cell sheet layering for creating three-dimensional tissue constructs.
    • Avoidance of complications associated with biodegradable scaffolds, such as inflammatory responses.

    Conclusions:

    • Cell sheet engineering provides a promising scaffold-free approach for tissue regeneration.
    • This method circumvents the limitations of traditional tissue engineering with biodegradable scaffolds.
    • Cell sheet engineering offers significant advantages for developing advanced regenerative therapies.