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Related Experiment Videos

Structural properties of poly C-scleroglucan complexes.

Marit Sletmoen1, Bjørn T Stokke

  • 1Biophysics and Medical Technology, Department of Physics, The Norwegian University of Science and Technology, NTNU, NO-7491 Trondheim, Norway.

Biopolymers
|July 14, 2005
PubMed
Summary

Polynucleotides like poly-cytidylic acid incorporate into linear (1,3)-beta-D-glucan structures, forming novel macromolecular complexes. These complexes exhibit increased density and persistence length compared to glucans alone.

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Area of Science:

  • Biochemistry
  • Polymer Science
  • Materials Science

Background:

  • (1,3)-beta-D-glucans can dissociate and reassociate into triple-helical structures.
  • Renaturation can yield a mix of circular and linear conformations.
  • Polynucleotides can form novel macromolecular complexes with (1,3)-beta-D-glucans.

Purpose of the Study:

  • To investigate the structural characteristics of (1,3)-beta-D-glucan-polynucleotide complexes.
  • To determine the incorporation site of polynucleotides within glucan structures.
  • To elucidate the molecular arrangement and properties of these novel complexes.

Main Methods:

  • Size exclusion chromatography (SEC) coupled with multi-angle laser-light scattering (MALLS) and refractive index (RI) detection.
  • Online ultraviolet (UV) detection for polynucleotide specific detection.

Related Experiment Videos

  • Atomic force microscopy (AFM) for structural topographs.

    • Main Results:

    • Poly-cytidylic acid (poly C) was found to incorporate into linear, but not circular, scleroglucan structures.
    • AFM confirmed a reduced fraction of circular structures upon poly C addition.
    • Poly C-scleroglucan complexes showed increased density and persistence length compared to linear scleroglucan.

    Conclusions:

    • Polynucleotides selectively bind to linear (1,3)-beta-D-glucan conformations.
    • The formation of these complexes alters the structural properties of the glucan.
    • The study provides insights into the molecular arrangement of these novel macromolecular complexes.