[Effects of nanotopography of bioactive surface on Pseudomonas fluorescence cell adhesion]

The nanoparticle-modified surfaces were built up by alternating deposition of oppositely charged Al2O3 and SiO2 nanoparticles (from 10 nm to 500 nm) solutions. The properties of these nanoparticle-modified surfaces and the controls were investigated by Atomic Force Microscope for topography analysis. Pseudomonas Fluorescence (PF) cell adhesion was evaluated by microscopic determination of the numbers of cells that adhered to the produced slides exposed to PF cell suspensions on static and dynamic condition. The results show that adhesion of PF to both surfaces readily increases with the time of exposition but the adhered numbers of PF on produced surfaces are considerably higher than that on controls in static condition. Cell morphologies on these nanoparticle-modified surfaces studied by inverted microscope show that the adhered PF on the produced surfaces are more in presence of clusters, which contributes more to the total adhering numbers in the late of cell adhesion assays. Meanwhile on controls the cells rarely attained confluence and had a single shape. The significant statistical correlation observed between nanoparticle-modified surfaces and control adds a new concept to the studies of substratum topography influence on cell behavior. The results suggest that nanoparticle-modified surfaces may enhance the interactions between PF cell and slides.