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Updated: Aug 17, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
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Published on: February 16, 2022

[Raman spectral study of nitrosyhemoglobin and several other hemoglobins]

Yan Cen1, Ren Zhang, Wen-hua Yao

  • 1Department of Physics, Fudan University, Shanghai 200433, China.

Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
|July 15, 2005
PubMed

The Raman spectra of nitrosyhemoglobin (NOHb), methemoglobin (MetHb), oxygenated hemoglobin (oxyHb), and deoxygenated hemoglobin(deoxyHb) were comparatively studied. The results show that the Raman spectra with 632.8 nm excitation can be used to detect the R state (oxyHb) to T state (deoxyHb) transition. The difference of NOHb and MetHb in their electron spin state was clearly demonstrated in their Raman spectra with 632.8 nm excitation, suggestingthat Raman spectra provide anew simple way to measure and distinguish NOHb and MetHb.

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