Related Experiment Videos
[Direct determination of ammonia in plasma with indophenol method]
Jian Deng1, Jin-sheng Xu, Hua Fan
1College of Chemistry and Chemical Engineering, Nanhua University, Hengyang 421001, China.
Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
|July 15, 2005
Summary
A new method accurately measures ammonia in plasma using an indophenol reagent. This sensitive technique is effective for diagnosing liver diseases and monitoring patient health.
Area of Science:
- Clinical Chemistry
- Analytical Chemistry
Context:
- Accurate ammonia quantification in plasma is crucial for diagnosing and managing various medical conditions, particularly liver diseases.
- Existing methods for ammonia determination can be complex or lack sensitivity.
Purpose:
- To develop and validate a direct, sensitive, and reliable method for quantifying ammonia in human plasma using the indophenol reagent.
Summary:
- A novel method utilizes the indophenol reagent in a phosphate buffer (pH 10.5-11.2) with sodium citrate anticoagulant for direct plasma ammonia determination.
- The method exhibits high sensitivity (detection limit 1.7 µmol/L), a wide linear range (0-1500 µmol/L), and excellent precision (RSD 1.13%).
- The reaction forms a stable blue compound with maximum absorption at 630 nm, showing minimal interference and high recovery rates (93.6%-101.6%).
Impact:
- This validated method offers a robust tool for clinical laboratories to assess plasma ammonia levels.
- It facilitates improved diagnosis and monitoring of liver diseases and other conditions associated with hyperammonemia.
- The method's simplicity and sensitivity contribute to more efficient and accurate patient care.