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[Study on the reaction mechanism of chloroacetanilide herbicides with urease using fluorescence spectrum and
Hui-jun Liu1, Xiu-Ming Zhan, Ke-bin Li
1Institute of Environmental Science, Zhejiang University, Hangzhou 310027, China.
Abstract:
The relationship between excess thermodynamic function and binding to urease of four chloroacetanilide herbicides was studied using high-performance liquid chromatography and fluorescence spectrum. The linear relationship between the composition of mobile phase of RP-HPLC and the capacity factor of chloroacetanilide herbicides has been obtained. The excess thermodynamic enthalpy (deltaH* ) of acetochlor, pretilachlor, butachlor and metolachlor was determined, and the binding constant K and the number of binding sites with urease were calculated. The relationship between excess thermodynamic function and the binding constant K was suggested.
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