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Published on: August 13, 2011
Enzymatic kinetic of cellulose hydrolysis: inhibition by ethanol and cellobiose
Rui M F Bezerra1, Albino A Dias
1CETAV-Departamento de Engenharia Biológica e Ambiental, Universidade de Trás-os-Montes e Alto Douro, Apartado 1013, 5001-911 Vila Real, Portugal. bezerra@utad.pt
Abstract:
The ethanol effect on the Trichoderma reesei cellulases was studied to quantify and clarify this inhibition type. To determine inhibition parameters of crude cellulase and purified exoglucanase Cel7A, integrated Michaelis-Menten equations were used assuming the presence of two inhibitors: cellobiose as the reaction product and ethanol as a possible bioproduct of cellulose fermentation. It was found that hydrolysis of cellulose by crude enzyme follows a model that considers noncompetitive inhibition by ethanol, whereas Cel7A is very slightly competitively inhibited. Crude cellulase is much more inhibited (K(iul) = K(icl) = 151.9 mM) than exoglucanase Cel7A (K(icl) = 1.6 x 1015 mM). Also, calculated inhibition constants showed that cellobiose inhibition is more potent than ethanol inhibition both for the crude enzyme as well as exoglucanase Cel7A.
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