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Related Experiment Videos

Lactate mapping in ischemic rat kidneys using 1H spectroscopic imaging.

F Terrier1, F Lazeyras, B M Frey

  • 1Institute of Diagnostic Radiology, University of Berne, Switzerland.

Investigative Radiology
|April 1, 1992
PubMed
Summary

Renal ischemia causes higher lactate buildup in the kidney medulla than the cortex. This study used 1H magnetic resonance (MR) spectroscopy to confirm this difference in lactate concentration during ischemia.

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Area of Science:

  • Biomedical imaging
  • Renal physiology
  • Metabolic studies

Background:

  • Renal ischemia leads to differential lactate accumulation between kidney medulla and cortex.
  • Previous biochemical studies indicated higher lactate levels in the medulla.
  • The precise spatial distribution of lactate during ischemia requires further investigation.

Purpose of the Study:

  • To confirm the difference in lactate concentration between renal medulla and cortex during ischemia.
  • To utilize 1H magnetic resonance (MR) spectroscopy for mapping intrarenal lactate distribution.
  • To investigate pathophysiologic mechanisms of renal ischemia.

Main Methods:

  • Applied four-dimensional and three-dimensional spectroscopic imaging techniques.
  • Investigated the left kidney in 10 rats following renal artery occlusion.

Related Experiment Videos

  • Obtained intrarenal lactate distribution maps during ischemic conditions.

    • Main Results:

    • Lactate concentration was significantly higher in the renal medulla compared to the cortex.
    • Medulla lactate levels measured at 17 +/- 4 mumol/g, cortex at 9 +/- 4 mumol/g.
    • Results were verified by subsequent chemical analysis.

    Conclusions:

    • Localized MR spectroscopy combined with imaging is a powerful tool for investigating pathophysiologic mechanisms.
    • This technique allows for correlating biochemical and morphologic information in vivo.
    • Confirms differential lactate accumulation in the kidney during ischemia.