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Updated: Jun 25, 2026
06:10
Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
Published on: September 2, 2019
[One major QTL mapping and physical map construction for rolled leaf in rice]
Yuan-Jian Shao1, Zong-Xiang Chen, Ya-Fang Zhang
1Agricultural College of Yangzhou University, Yangzhou 225009, China.
Yi Chuan Xue Bao = Acta Genetica Sinica
|July 16, 2005
Summary
Researchers mapped a major rolled leaf QTL (rl8) in rice, explaining up to 33% of phenotypic variation. This finding aids in understanding leaf rolling and improving marker-assisted selection for plant breeding.
Area of Science:
- Plant genetics
- Quantitative trait loci (QTL) analysis
- Rice genomics
Context:
- Leaf rolling is a significant trait affecting rice yield and quality.
- Understanding the genetic basis of leaf rolling is crucial for crop improvement.
- Previous studies have identified quantitative trait loci (QTLs) associated with leaf rolling.
Purpose:
- To map and characterize quantitative trait loci (QTLs) associated with rolled leaf in rice.
- To identify major QTLs controlling leaf rolling and estimate their effects.
- To construct an integrated physical and genetic map for fine mapping.
Summary:
- A clonally propagated F2 population from a cross between Qimiaoxiang (unrolling) and 91SP068 (medium rolling) rice varieties was used.
- One major QTL, rl8, derived from 91SP068, was mapped to chromosome 5 between SSR markers RM6954 and RM6841.
- rl8 explained 20%–33% of phenotypic variation, with significant additive and dominance effects.
- An integrated physical and genetic map of the rl8 region was constructed, with a physical distance of 542 kb.
Impact:
- Facilitates map-based cloning of the rolled leaf gene.
- Improves the efficiency of marker-assisted selection in rice breeding.
- Contributes to a deeper understanding of the genetic architecture of leaf rolling in plants.
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02:27Trihybrid Crosses
Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal chance to...
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal chance to...