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Updated: Aug 17, 2026
Bergmeyer Glucose Quantification for Microbiological Samples
Published on: January 17, 2025
Assessment of trueness of glucose measurement instruments with different specimen matrices
Hubert W Vesper1, Enada Archibold, Gary L Myers
1Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Hwy NE MS F25, Atlanta, GA 30341, United States. HVesper@cdc.gov
Background:
The trueness of glucose monitors is commonly assessed using whole-blood samples and a clinical analyzer as a comparison method. In this study, the effect of specimen matrix on trueness of one clinical analyzer and one glucose monitor was compared with a gas chromatography-mass spectrometry (GC/MS) reference method, using split-sample comparison with capillary whole blood (CWB), venous whole blood (VWB), and plasma (PL).
Methods:
CWB was analyzed by the glucose monitor and the GC/MS reference method. VWB was analyzed by the glucose monitor, clinical analyzer, and the GC/MS method. PL was analyzed by the clinical analyzer and the GC/MS reference method.
Results:
For the glucose monitor, the bias was 0.4% and -18.2% for CWB and VWB, respectively. The clinical analyzer had a bias of -25.4% for VWB and -12.0% for PL and a proportional bias was detected in both specimens. Using the clinical analyzer as a comparison method, the glucose monitor had a proportional bias of -9.8%.
Conclusion:
The trueness of clinical analyzers can be affected by the specimen matrix that needs to be assessed before they are used as comparison method to assess trueness of glucose monitors.
