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Clonal expanded TCR Vbeta T cells in patients with APL
Yangqiu Li1, Shaohua Chen, Lijian Yang
1Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, China. yangquili@hotmail.com
Hematology (Amsterdam, Netherlands)
|July 16, 2005
Summary
This study found skewed distribution and clonal expansion of T-cell receptor Vbeta subfamily T-cells in acute promyelocytic leukemia (APL) patients. This suggests a specific anti-leukemic immune response in APL.
Area of Science:
- Immunology
- Hematology
- Molecular Biology
Background:
- T-cell receptor (TCR) Vbeta gene repertoire and clonality are crucial in leukemia and solid tumors.
- Limited research exists on leukemia-associated oligoclonal T-cells in acute promyelocytic leukemia (APL).
Purpose of the Study:
- To investigate the distribution and clonal expansion of TCR Vbeta subfamily T-cells in patients with t(15;17) APL.
- To analyze the CDR3 of TCR Vbeta24 subfamily genes in APL patients.
Main Methods:
- Analysis of peripheral blood mononuclear cells from 17 APL patients and 10 healthy controls.
- Utilized Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Genescan analysis to assay CDR3 size of TCR Vbeta genes.
Main Results:
- The number of expressed Vbeta subfamilies varied among APL patients (2-21 subfamilies).
- Frequently expressed Vbeta subfamilies included Vbeta2 (64.7%), Vbeta15 (58.8%), Vbeta3, and Vbeta5 (47.1%).
- Clonally expanded T-cells were identified in most APL cases, particularly in Vbeta10, Vbeta23, Vbeta3, and Vbeta21 subfamilies.
Conclusions:
- Skewed distribution and clonal expansion of TCR Vbeta subfamily T-cells are evident in APL patients.
- T-cell clonal expansion may represent a host anti-leukemic immune response to leukemia-associated antigens.